Review



cell culture wild type wt t84  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    ATCC cell culture wild type wt t84
    Cell Culture Wild Type Wt T84, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+wild+type+wt+t84/T84/pm41525418-375-3-9
    Average 96 stars, based on 1520 article reviews
    cell culture wild type wt t84 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Imaging:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Microscopy:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    DNA Purification:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Gel Extraction:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Cytotoxicity Assay:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Sequencing:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Cell Culture:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Article Title: Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cells.
    Article Snippet: .. Cell lines and cell culture Wild type (WT) T84 (ATCC CCL-248) as well as T84 knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). .. Lenti-X HEK293T cells (Takara, 632180) were grown in DMEM (Gibco #31965).

    Knock-Out:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Article Title: Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cells.
    Article Snippet: .. Cell lines and cell culture Wild type (WT) T84 (ATCC CCL-248) as well as T84 knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). .. Lenti-X HEK293T cells (Takara, 632180) were grown in DMEM (Gibco #31965).

    Modification:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Article Title: Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cells.
    Article Snippet: .. Cell lines and cell culture Wild type (WT) T84 (ATCC CCL-248) as well as T84 knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). .. Lenti-X HEK293T cells (Takara, 632180) were grown in DMEM (Gibco #31965).



    Similar Products

    cell culture wild type wt t84  (ATCC)
    96
    ATCC cell culture wild type wt t84
    Cell Culture Wild Type Wt T84, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+wild+type+wt+t84/T84/pm41525418-375-3-9
    Average 96 stars, based on 1 article reviews
    cell culture wild type wt t84 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results